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藻类群落结构扫描成像分析系统—浮标版CytoBuoy
日期:2017-03-27 14:21:35
主要功能




1. 专业分析浮游植物细胞,同时具备传统流式细胞仪经典功能1.jpg
2. 可以扫描记录各种光学信号(散射、荧光)的动态变化
3. 可实现高频、原位分析水体微生物群落及优势种变化
4. 可在完整的藻类粒径谱范围内对生物量进行线性评估
5. 可直接分析大尺寸范围的浮游藻类、团体结构,可现场分析微囊藻群体结构变化
6. 可调式PMT可根据检测粒径大小调节检测器灵敏度
7. 流动成像技术可对感兴趣感兴趣的聚群进行圈门设定后专门拍照
8. 脉冲信号指纹图谱技术,圈门直观方便,更真实反应细胞形态
9. 水下测量(CytoSub)可在整个真光层分析浮游植物动态
10. 可整合入浮标中或其它载体上进行在线监测,可配合CTD对水体做剖面测量
11.实现实验室远程控制基站式自动在线监测,可实现完全自动检测,无人值守在线监测






测量参数




光学参数:      前向散射FWS、侧向散射SWS,荧光散射FLR、 FLY、 FLO
形态参数:      能同时获得包括细胞和颗粒形态物理特性(数量、长度、大小、形态、粒度、色素、峰数等)、群体特征、脉冲图谱等在内的9个拓扑学指标及最少45组参数
绝对计数:自然水体总颗粒计数,圈门后可集群计数及浓度计算,可实现链状藻单细胞数计数功能
其他测量参数:分析体积、进样速率等






应用领域




1. 海洋生态学与淡水生态学


2. 流域监测与管理


3. 海洋学与湖沼学


4. 有害藻华(HABs)预警


5. 微藻生物技术


6. 河流、水库、湖泊、海洋的监测与管理


7. 监测与管理


8. 水源地、水厂、污水处理厂的水质监测


9. 富营养化研究


10. 藻类环境生物学


11. 水产养殖








选购指南:




一、便携式浮游植物流式细胞仪CytoSense

系统组成:



流式细胞仪分析主机:相干高质量连续固态激光器,标配波长488nm, 可选波长445nm、635nm、640nm、660nm等最多可配置7个检测器(检测通道含FWS L+R、SWS、YF、RF、OF)。
野外便携式外壳:仪器采用碳素纤维外壳,防溅水设计,更轻便(<15kg),整机安装于轻质铝质框,带高质量防震垫。包装于便携式航空箱内。
数据分析系统:含便携式笔记本电脑,预装数据采集软件CytoUSB,和数据分析软件CytoClus
批量处理数据分析软件EasyClus : 需购买MatLab软件配合使用
高速流动成像模块:可选。

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      便携式浮游植物流式细胞仪                   Easyclus 粒径分布图                      Easyclus 散点图
系统组成:



主机:浅水版Cytosub (水下20米),含CytoSense所有基本配置

浮标模块:包括浮标、太阳能电池板、充电电池、浮标灯、电子系统、无线传输装置和采样管防水连接器等。根据用户需要,也可扩展为易拆卸浮标模块,这样用户可以非常方便的在CytoSense(室内用)和CytoBuoy(在线监测)间转换。
注意:野外在线监测时不仅仅限于以浮标作为平台,其他平台也可,只要可以具备放置CytoSense的空间及供电即可。同时,增加Bacterial staining module,可实现水体异养微生物自动染色和在线分析,可在线检测藻类、细菌、浮游动物及沉积物等颗粒。具体信息请来电咨询。


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                       CytoBuoy 浮体

CytoBuoy通讯模式:无线通讯



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三、水下浮游植物流式细胞仪——CytoSub 


主机:台式机CytoSense是防溅水设计,可以在野外使用,但不能水下使用。CytoSense加上一个水下模块(SUB MODULE)就组成了水下式流式细胞仪CytoSub。
水下模块:一个耐受200 m水深压力的防水外壳,阀门和进样环路部分(包括循环泵),电子控制单元,数采,水下连接器和支架。

cytosub 主机.jpg7.jpg





               Cytosub 主机                         CytoSense CytoSub 转换
工作模式一:AUV搭载




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利用英国国家海洋中心AutoSubAUV搭载CytoSub

工作模式二:水下垂直剖面分析




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                             与CTD结合一起测量

注意:此外,水下型浮游植物流式细胞仪CytoSub可应用于浮标,Ferrybox等监测平台,在垂直剖面不同层位获取浮游植物生物量信息,对研究微囊藻沉浮机制,浮游动物、水文、水质等因素对浮游植物生态位影响提供数据依据。

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                           CytoSense 检测对象

产地:荷兰 CytoBuoy



参考文献
数据来源: Cytometry ,  Goolge scholar等,截至2016年,共收集相关文献近100篇。

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