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浮游植物分类荧光仪——PHYTO-PAM-II
日期:2017-01-04 22:12:05

主要功能

●对自然水体中的蓝藻、绿藻、硅/甲藻和隐藻自动分类(定性)

●自动测量水样中蓝藻、绿藻、硅/甲藻和隐藻的叶绿素 a 含量(定量)和总叶绿素 a 含量

●一杯自然水样,同时获得蓝藻、绿藻、硅/甲藻和隐藻的光合活性:

●光合效率和光合速率(相对电子传递速率)

●快速光曲线并进行拟合

●藻类的潜在最大光合效率

●藻类的光保护能力

●藻类耐受强光的能力

●用户可做自己的参考光谱


测量参数

Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和叶绿素含量等

 

应用领域

●主要用于水生生物学、水域生态学、海洋学、湖沼学、水质预警、微藻生理学、微藻抗逆性等领域,对于了解自然水样中藻类种群的动态变化、水华预警、野外水体中光合作用的时空变化、校正初级生产力的计算等有较大帮助。

●特别适于浮游植物动力学研究和有害水华的早期预警。

 

主要技术参数

●主控单元:金属外壳,包含所有的光电元件及样品测量室

●测量光: LED,440 nm,480 nm,540 nm,590 nm 和 625 nm,5 波长脉冲调制测量光,2 档强度设置,8 档调制频率设置, 3 档测量光自动高频设置

●光化光:板载多波长 LED 阵列,440 nm,480 nm,540 nm,590 nm,625 nm 和 420-640 nm(白光),提供持续光化光,最高可达 1400 μmol m-2 s-1,快速动力学闪光高达 7000 μmol m-2 s-1,饱和脉冲最高可达 5000 μmol m-2 s-1

●远红光:725 nm LED

●信号检测:基于 H-10720 光电传感器模块的光电倍增检测器

●标准检测过滤器:> 650 nm 长通滤光片

●高分辨率的光电倍增管,叶绿素浓度检测限低至 0.1μg/L,适用野外采集的藻浓度很稀的样品

 

升级的技术特点

●可提供 5 种波长的脉冲调制测量光和光化光

●板载 LED 阵列芯片技术

●可实时进行四种藻的分类

●可进行标准 PAM 测量及不同波长强光化光诱导的毫秒级荧光上升动力学分析

●可测定光系统 II 功能性捕光截面积

●内置自动测量程序,易于操作

 

PHYTO-PAM-II vs PHYTO-PAM

  便携式 PHYTO-PAM-II

  PHYTO-PAM

  5 种不同波长的测量光用于生物体内不同类型的天线色素荧光的激发

  4 种不同波长的光

  4 种色素类型的在线分类

  3 种色素类型的在线分类

  6 种波长的光化光

  1 种波长的光化光

  可以分别测量绿藻,蓝藻,硅/甲藻以及含有藻红蛋白的有机体,如隐藻不同波长下 PSII 的活性

  无该功能

  紧凑型设计

  需要组装

  增加了快速动力学操作模式,可通过强光化光脉冲,测量不同波长的 O- I1 荧光上升动力学曲线

  无该功能

  通过测定不同光质和光合生物色素复合体,获得光系统 II 功能性捕光截面积的信息即 σPSII

  无该功能

  通过 FluoRed 荧光标准将参考光谱校准标准化

  无该功能

  获得的参考光谱可在不同设备及用户间互换使用

  参考光谱不能互换

  

选购指南

一、实验室测量基本款

系统组成:实验室版主机,激发检测单元,悬浮液的光学单元,球形光量子传感器,工作台,软件等

注意:新版 PHYTO-PAM-II 必须配置光量子传感器,校准光强后参考光谱可在不同仪器间通用

Phyto-PAM II laboratory version.jpg

实验室测量基本款

  

二、野外便携紧凑款

系统组成:紧凑型主机,球形光量子传感器,软件等

注意:新版 PHYTO-PAM-II 必须配置光量子传感器,校准光强后参考光谱可在不同仪器间通用

Phytho-PAMM-II_071_1-small-2.jpg

便携式紧凑款


Channels.jpg

algae.jpg

Reference.jpg

五种测量光通道

区分四种藻类

通用型参考光谱

软件界面7.png

light-curve.jpg

Fast-Kinetics.jpg

测量慢速动力学曲线

淬灭分析,暗弛豫分析

测量快速光曲线

光响应曲线

测量快速动力学曲线

分析PSII功能性捕光截面


三、其他可选附件

1,WATER-S:搅拌器(推荐选配),利用内置电池驱动的马达带动搅拌棒旋转,对样品杯里的悬浮液进行搅拌。需配置搅拌棒 WATER-R。

2,WATER-R:搅拌棒(标配10根)

 

产地:德国WALZ


参考文献

数据来源:光合作用文献 Endnote 数据库,更新至 2021 年 1 月,文献数量超过 10000 篇

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