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OX1LP Dissolved Oxygen OX1LP溶解氧测量仪——可测量任何液态样本中的氧气含量
日期:2020-06-03 15:02:13

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简介


OX1LP溶解氧测量仪使用极谱溶解氧电极,可用于测量水相中的光合作用和/或呼吸作用,适用于藻类、植物、动物和细胞器(叶绿体和线粒体)的悬浮液。还可用于监测在水相中产生或消耗氧气的化学和生化反应。溶解氧包配有仪器、附件、软件和接口。

可提供从100ul(流通)到50ml不同体积的DO电极。水套反应杯内的氧电极可用于测量生物样品或酶反应的O2消耗(或产生)。


采用简单的两点校准, 可在相同的温度下进行校准和实验,或者可以在软件中对不同温度下的测量进行简单的温度校正。随机附校准所需的所有附件。





测量对象


v 藻类光合作用与呼吸作用

v 细菌呼吸

v 微生物呼吸

v 小型水生动物的呼吸

v 水生昆虫和幼虫呼吸

v 线粒体呼吸

v 叶绿体O2的产生

v 细胞悬浮呼吸

v 植物细胞悬浮光合作用



应用领域


v 水生植物光合氧代谢的测定

v 抑制剂对线粒体电子传递的影响

v 光依赖性叶绿体电子输运分析

v 温度对水生动物摄氧量的影响

v 酶动力学耗氧研究





基本组件


溶解氧反应杯电极(1,2.5,4,6,30,50ml)

反应杯电极放大器

磁力搅拌器

搅拌棒和O形圈

KCl填充液

膜材料和滤纸

水套用10英尺Excelon管

LabQuest迷你数据接口

Logger Pro软件

定制安装软件

操作手册


可选组件:


向反应杯中添加代谢物的注射器; 10微升,50微升,100微升

样品温度测量用热敏电阻

校准LED光源

Q-Box坚固外壳,用于储存和运输所有部件








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DO电极,带磁力搅拌器和LED光源





不同体积的溶解氧反应杯电极


OX1LP-1 (1ml 有机玻璃)

OX1LP-2.5 (2.5ml 玻璃 或有机玻璃)

OX1LP-4 (4ml 有机玻璃)

OX1LP-6 (6ml 玻璃)

OX1LP-30 (30ml 有机玻璃)

OX1LP-50 (50ml有机玻璃或玻璃)




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软件界面及数据


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客户评价


“I have tested my new Qubit system with isolated oyster mitochondria, and was extremely pleased with the results. I can’t believe I was suffering for the whole year with my old chamber. I have tried three different systems based on Clarke-type electrodes including this one, and this is by far the most convenient for use with small sample volumes and gives a very clean, repeatable trace with the best signal to noise ratio I ever had with micro-chambers.”

From Dr. Inna Sokolova, Assistant Professor, Biology Department, University of North Carolina at Charlotte

“我用分离的牡蛎线粒体测试了Qubit系统,对结果非常满意。我真不敢相信忍受了整整一年旧培养室。我尝试了三种不同类型的克拉克电极,包括这一种,这是迄今为止测量小样本很方便的系统,提供了一种非常干净、可重复的轨迹,且在微型培养室中信噪比很好。”

 ——北卡罗来纳大学生物系助理教授 Dr. Inna Sokolova


发表文献


· Lamarre SG et al. Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). (2019Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051) 

· Sirikhachornkit et al. Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient Mutants. J. Microbiol. Biotechnol.  26(5), 854–866 (2016)

· Croston, Tara L., et al. Functional deficiencies of subsarcolemmal mitochondria in the type 2 diabetic human heart. American Journal of Physiology-Heart and Circulatory Physiology 307.1 H54-H65 (2014)

· Sussarellu, Rossana, et al. Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569 (2013).

· Dickinson, Gary H., et al. Environmental salinity modulates the effects of elevated CO2 levels on juvenile hard-shell clams, Mercenaria mercenaria. The Journal of experimental biology 216.14 2607-2618 (2013).

· Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O2 by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

· Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

· Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

· Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798(2008).

· Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)

· Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis inChlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)

· Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

 

 

产地:加拿大



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