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草业科学专用的叶绿素荧光成像系统Weed-PAM
日期:2018-05-09 14:53:00

草业科学-叶绿素荧光成像系统Weed-PAM

基于功能强大的IMAG-PAM,改进升级后适用于野外杂草,草坪等光合作用研究

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主要功能

l 成像功能:对FtFoFmFv/FmFFm’Y(II)Y(NO)Y(NPQ)NPQqNqPqLETRAbs.NIRRed等至少17种参数进行成像分析。测定调节性能量耗散Y(NPQ),反映植物光保护能力,测定非调节性能量耗散Y(NO),反映植物光损伤程度。

l 程序测量功能:可程序测量荧光诱导曲线、快速光曲线和暗弛豫,也可手动测量;在测量过程中能自动分析所有荧光参数的变化趋势

l AOI功能:可在测量前或测量后任意选择感兴趣的区域(AOI),程序将自动对选择的AOI的数据进行变化趋势分析,并在报告文件中显示相关AOI的数据。所有报告文件中显示的数据都可导出到EXCEL文件中。

l 成像异质性分析功能:对任意参数任意时间的成像,可在图像上任意选取两点,软件自动对两点间的数据进行横向异质性分析,并可导出到EXCEL文件中。

l 成像数据范围分析功能:对任意参数任意时间的成像,可分析任意两个荧光数值之间有多少个像素点,多少面积(cm2)。

l 突变株筛选功能:可跟据成像结果快速筛选光合、产氢/油、抗逆(抗盐、抗旱、抗病等)等突变株

l 吸光系数测量功能:快速测量叶片的吸光系数。吸光系数测量光源: 16个红光(650 nm)和16个近红外(780 nmLED,用于测量植物叶片或藻类样品PAR吸光系数。

成像参数

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

技术参数

Maxi探头(蓝光版)蓝色LED,450 nm


Maxi探头(红光版)红色LED650 nm


Mini探头(蓝光版蓝色LED460 nm

 

Mini探头(红光版红色LED620 nm


应用领域

光合作用研究:可以在相同的条件下同时对大量样品进行成像

l 植物病理学:病斑部位(包括肉眼不可见时)成像以及病斑扩散的时空动力学

l 植物胁迫生 理学:肉眼不可见胁迫损伤的早期检测

l 遗传育种:出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

l 突变株筛选:快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

l 分子生物学:宏观水平上检测样品的绿色荧光蛋白(GFP)荧光

l 其它多种扩展研究



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参考文献

数据来源:光合作用文献 Endnote 数据库,更新至 2021 年 1 月,文献数量超过 10000 篇

原始数据来源:Google Scholar

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